Polyethylene glycol precipitation: proceed with care
نویسندگان
چکیده
منابع مشابه
Polyethylene glycol precipitation as a screening method for macroprolactinemia.
To the Editor: Vieira et al. (1) used the Wallac Delfia immunofluorometric assay to demonstrate that macroprolactin is a common cause of apparent hyperprolactinemia, and this confirms our experience (2) and that of others (3). However, their data validating the polyethylene glycol (PEG) precipitation as a screening method for detecting macroprolactinemia are substantially different than ours (2...
متن کاملPrecipitation of DNA by polyethylene glycol and ethanol.
The use of polyethylene glycol (PEG) for precipitation of DNA has been described in the literature (1-6) . We present here conditions for precipitation of DNA by PEG that give improved recoveries of DNA so that precipitation by PEG is comparable to that by ethanol, in terms of recovery, over a wide range of DNA concentrations. The problem of precipitation of lowmolecular-weight DNA fragments by...
متن کاملMacroprolactin detection by precipitation with protein A-sepharose: a rapid screening method compared with polyethylene glycol precipitation.
within 21 days. The likelihood ratio for a negative result [(1 sensitivity)/specificity] was 0.59 for birth within 7 days, 0.68 for birth within 14 days, and 0.53 for birth within 21 days. The data in Table 1 are similar to those obtained in previous studies using the ELISA-based assay. Joffe et al. (1 ) reported a NPV of 99.6% and a PPV of 9.1% for 243 patients who delivered within 7 days with...
متن کاملSize fractionation of double-stranded DNA by precipitation with polyethylene glycol.
We show that DNA molecules of differing molecular mass are separable by selective precipitation with polyethylene glycol (PEG+.. Higher molecular mass DNA precipitates at lower PEG concentrations than lower molecular mass DNA. Double-stranded DNA can be fractionated at least in the range of 3 times 10-7 to 1 times 10-5 daltons. The effects on PEG concentration, sodium chloride concentration, DN...
متن کاملAssessment of a method for measuring serum thyroxine by radioimmunoassay, with use of polyethylene glycol precipitation.
We assessed the efficacy of a new thyroxine radioimmunoassay kit (Abott) in which polyethylene glycol is used to separate bound from free hormone. Mean serum thyroxine was 88 +/- 15 (+/-SD) microgram/liter for 96 normal persons. Results for hypothyroid and hyperthyroid persons were clearly separated from those for normal individuals. Women taking oral contraceptive preparations showed variable ...
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ژورنال
عنوان ژورنال: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
سال: 2008
ISSN: 0004-5632,1758-1001
DOI: 10.1258/acb.2008.007262